Fig. 6
From: Dissection of MAPK signaling specificity through protein engineering in a developmental context
MKK5 and MKK7 activity and localization in the stomatal lineage can be reciprocally rewired. a–b Paired micrographs of representative major phenotype (left) and subcellular localization (right), specific MKK-YFP variants diagramed above. Black brackets mark clusters; YFP is in green and cell outlines in magenta; white arrowheads point to mitochondrial localization. a N7-MKK5DD-C7 mimics MKK7ED in that it produces clusters and can localize to mitochondria. b N5-MKK7ED-C5 mimics MKK5DD in that it has a WT phenotype and localizes in the cytoplasm. Scale bars are 50 μm in phenotype images and 10 μm for localization images. c Quantification of phenotypes (a–b). d SPCH stage inhibition of lineage initiation. Error bars in (c) and (d) correspond to 95% confidence interval. e In vitro kinase activity towards kinase inactive MPK3 and MPK6. Kinase assays were performed in triplicates, normalized to unphosphorylated KI-MPK and averaged; error bars represent standard errors