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Fig. 2 | BMC Plant Biology

Fig. 2

From: Dissection of MAPK signaling specificity through protein engineering in a developmental context

Fig. 2

Differences phenotypic output between MKK5 and MKK7. a Micrographs of phenotypes resulting from FAMA-stage expression of MKK5DD and MKK7ED: precursors exit the stomatal lineage and do not make GCs (arrowheads); Normal (single stomata comprised of two GCs) or small (2–3) or large (4+) clusters of adjacent stomata resulting from overproliferation of precursors before they become GCs. Scale bars are 10 μm. b Quantification of phenotypes, percentage of seedlings showing one of four phenotypes. >19 independent transformants were scored per genotype and stage (Ns reported in Table 1). 35Spro:YFP was used as a negative control (see Methods). Error bars correspond to 95% confidence intervals

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