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Fig. 1 | BMC Plant Biology

Fig. 1

From: Analyses of starch biosynthetic protein complexes and starch properties from developing mutant rice seeds with minimal starch synthase activities

Fig. 1

Zymograms of soluble starch biosynthetic enzyme activities. Native-PAGE/activity staining of starch synthases (SSs; Panel a), branching enzymes (BEs; Panel b), and debranching enzymes (DBEs; Panel c) using developing endosperm of double mutant, parental mutant, and wild-type lines. Black arrowheads and brackets indicate SSI, SSIIIa, BEI, BEIIa, BEIIb, ISA (isoamylase), PUL (pullulanase), and Pho1 (phosphorylase) activity bands. Gray arrowheads in A indicate glycosyl hydrolase or transferase activities. Soluble protein extracts were prepared from developing endosperm at 12 days after flowering. The volumes of crude extract applied to the native gels in section ‘a’ were 2-fold and 4-fold greater than those applied in sections ‘b’ and ‘c’, respectively

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