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Fig. 1 | BMC Plant Biology

Fig. 1

From: Low pyrrolizidine alkaloid levels in perennial ryegrass is associated with the absence of a homospermidine synthase gene

Fig. 1

Genotyping assays for the presence (HSS1+) or absence (HSS1-) of the LpHSS1 gene. a Location of primers within the LpHSS1 gene. Black arrow bars represent exon locations of the LpHSS1 gene and the position of the start (ATG) and stop (TGA) codons are indicated. Blue bars represent PCR products from various primer combinations used to genotype plants. Red bars represent the F9 + R8 and F5 + R8 PCR products which were developed into a multiplex PCR assay for the genotyping of the entire association mapping population. Numbers given on blue and red bars are PCR product sizes. b Screening plants from the association mapping population for LpHSS1 using the F5 + F9 + R8 multiplex assay. The positive control product from the endogenous cab gene confirms a successful PCR when no LpHSS1 gene product is amplified.

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BMC Plant Biology

ISSN: 1471-2229

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