Fig. 1

Phenotypes of transgenic plants (RNAi-20, Ox-2, Ox-22 and Ox-45) along with WT grown at different temperatures. Germinated seeds were grown in growth chambers at 22 °C (a) or 30 °C (b) under 80 μmol•m^− 2 s^− 1 light intensity. Transcripts of OsNOA1 were determined by real-time PCR (n = 4) under both temperatures (c), and the mean values of NOA1 protein in two biological sets grown at 22 °C were detected by TMT-LC-MS/MS (d). Contents of soluble proteins (e) and chlorophyll (f) were determined (n = 4). Plots of abundance of Rubisco and chlorophyll against OsNOA1 expression level were shown in the inset of (e and f), respectively. Data are means ±SD of n biological replicates and representative of three independent experiments. For (e and f), data significances were determined by DMRT (Duncan’s new Multiple Range Test). Differences were considered significant at the level of P < 0.01