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Table 2 Comparison results of clean sequences on reference genomes

From: Transcriptomic analysis reveals overdominance playing a critical role in nicotine heterosis in Nicotiana tabacum L.

Sample

Clean reads

Total mapped

Multiple mapped

Uniquely mapped

Reads map to +

Reads map to -

Non-splice reads

Splice reads

VA116

69282386

56937342 (82.18%)

1149403 (1.66%)

55787939 (80.52%)

27915758 (40.29%)

27872181 (40.23%)

39876131 (57.56%)

15911808 (22.97%)

Basma

76348022

63090417 (82.64%)

1516187 (1.99%)

61574230 (80.65%)

30785508 (40.32%)

30788722 (40.33%)

40938897 (53.62%)

20635333 (27.03%)

F1

66926392

53935066 (80.59%)

1303116 (1.95%)

52631950 (78.64%)

26330586 (39.34%)

26301364 (39.3%)

34462915 (51.49%)

18169035 (27.15%)

  1. (1) Total mapped represents the total amount of sequencing sequences that can be mapped to the genome. (2) Multiple mapped represents the total amount of sequencing sequences with multiple alignment positions on the reference sequence. (3) Uniquely mapped represents the total amount of sequencing sequences with unique alignment position on the reference sequence. (4) Reads map to ‘+’, Reads map to ‘-’ represent the number of sequencing sequence that aligne to positive and negative chains on the genome