Fig. 3
From: Sequence variation and functional analysis of a FRIGIDA orthologue (BnaA3.FRI) in Brassica napus
Expression and subcellular localization analysis of BnaA3.FRI. a Expression of BnaA3.FRI between different crop types of B. napus. ‘+V’ indicates tissues after vernalization, ‘-V’, indicates tissues before vernalization. b qRT-PCR analysis of BnaA3.FRI gene expression in various tissues, (c-g) BnaA3.FRI promoter-driven GUS expression, seedling (c), rosette leaf (d), flowers (e), stem (f), and silique (g). Bars = 1 mm. h Subcellular localization of BnaA3.FRI. Co-expression of YFP-BnaA3.FRI and CFP-GHD7 fusion proteins in leaf epidermal cells of tobacco. From left to right: YFP fluorescence, CFP fluorescence showing the nuclei, bright-field (BF), merged image of YFP/CFP, and merged image of YFP/CFP/BF. Bar = 20 μm