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Fig. 6 | BMC Plant Biology

Fig. 6

From: Variable-angle epifluorescence microscopy characterizes protein dynamics in the vicinity of plasma membrane in plant cells

Fig. 6

Single particle tracking and computational analysis on puncta of SKU5-GFP captured with VAEM. a A representative frame of time-lapse images for SKU5-GFP tagged puncta; b Trajectory of tracked puncta of SKU5-GFP-tagged puncta in panel (a); c The tracked SKU5-GFP tagged puncta were skewed to two populations in the histogram analysis based on fluorescence intensity after Gaussian fitting, implying different clustering pattern for the two populations; d TyrA23 treatment significantly altered the proportions of the two populations (P < 0.05); e The SKU5-GFP puncta were aligned to two populations in the histogram analysis based on life time after exponential fitting, implying different association states of the two populations; f The lifetime for two populations of SKU5-GFP puncta did not change significantly upon TyrA23 treatment (P > 0.05). Bars = 50 μm; P < 0.05, student t-test was carried out to examine the significance for the differences in the proportions between the two populations of SKU5-GFP puncta after tyrA23 treatment; Interval between frames = 200 ms

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