Fig. 5

Comparative analysis on the PM subcompartmentation using LSCM and VAEM. To obtain a detailed view of the membrane domains, LSCM was firstly used to image the upper surface plane for cotyledon epidermal cells; VAEM and kymograph analysis were utilized to capture protein dynamics on the PM. a AtFlot1a displayed obviously uneven localization under LSCM and high lateral motility under VAEM, arrows indicate deviations in kymograph curves; (b-d) 35S:StREM, 35S:AtREM1.2, pREM1.2:AtREM1.2 showed diffused but uniformly distributed fluorescence labeling of the PM, while they formed puncta showing low lateral motility under VAEM; (e-f) pSKU5:SKU5-GFP and pCOBRA:COBRA-GFP demonstrated similar distribution patterns to those of REMs, but displayed relatively higher lateral motility. Bars = 50 μm (LSCM), 10 μm (VAEM); Interval between frames = 200 ms