Fig. 3

Co-localization analysis on proteins of interest using VAEM-based single particle tracking. a Transgenic Arabidopsis line co-expressing CLC-GFP / DRP1C-mOrange, GFP-FLOT1a / mCherry-FLOT1a and CLC-GFP / mCherry-FLOT1a were subjected to VAEM followed by single particle tracking and calculation on the distance between the captured punctate structures labelled by dual colors. b We classified the resulting distances into three categories: (i) colocalized: a distance between two centers that was below the resolution limit of the objective lens (0.24 μm in this study); (ii) associated: a distance less than the sum of the radii of two punctate structures (< 0.96 μm in this study); and (iii) independent: a distance larger than the sum of the radii of two punctate structures (> 0.96 μm). D stood for the distance between the centers of two adjacent puncta for analysis. c Percentages of co-localized, associated and independent puncta for the protein candidates