Fig. 4

Identification of the PPVres locus variants mediating PPV resistance in apricot. a Variant filtering of SNPs and small INDELs within the PPVres locus called using 24 apricot cultivars and 2 apricot relatives WGS. b Positions of filtered variants in the peach syntenic region (green lines) corresponding to the PPVres locus. MATHd genes cluster is indicated and peach MATHd genes absent in the apricot genome appear grey colored. Variants in ParP-3 and ParP-4 (putative orthologs of ppa022254m and ppb0221 95 m) are detailed below. The 5-bp deletion causing a frameshift mutation is labeled with an asterisk. c ParP-4 CDS and predicted amino acid sequences for the resistant (R) and susceptible (S) alleles. The 5-bp deletion (green boxed) leads to a premature stop-codon (red boxed) in the R-allele. qRT-PCR primer positions were indicated by arrows (blue, forward R-allele-specific; red, forward S-allele-specific; black, reverse). (d) ParP-4 allele-specific PCR-genotyping in 4 PPV resistant and 5 PPV susceptible apricot cultivars (GO: ‘Goldrich’; HA: ‘Harlayne’; OR: ‘Orange Red’; ST: ‘Stella’; CA: ‘Canino’; KA: ‘Katy’; CU: ‘Currot’; GI: ‘Ginesta’; MI: ‘Mitger’)