Fig. 4
MdY3IP1 accumulates less ROS than the control in Arabidopsis leaves. a DAB staining for H2O2 in the leaves of the control and MdY3IP1 transgenic Arabidopsis grown with or without a 200 mM NaCl. b DAB staining intensity as determined with imageJ software. c NBT staining for superoxide in the leaves of the control and MdY3IP1 transgenic Arabidopsis grown with or without a 200 mM NaCl. d NBT staining intensity as determined with imageJ software. e DCFH-DA staining for H2O2 in the leaf protoplasts of the control and MdY3IP1 transgenic Arabidopsis treated with or without 200 mM NaCl. Bars = 100 mm. f DCFH-DA staining intensity as determined with imageJ software. Three independent experiments were done with similar results, each with three replicates, and each replicate with 20 to 30 protoplasts. Note: In b, d f, data are shown as the mean ± SE, based on more than nine replicates. Statistical significance was determined using Student’s t test. *P < 0.01; **P < 0.001