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Fig. 2 | BMC Plant Biology

Fig. 2

From: Ectopic expression of the apple nucleus-encoded thylakoid protein MdY3IP1 triggers early-flowering and enhanced salt-tolerance in Arabidopsis thaliana

Fig. 2

Photosynthetic sugar metabolism process is accelerated in MdY3IP1 transgenic Arabidopsis. a qPCR analysis of AtSOC1, AtFT and AtFLC transcript levels in the control and MdY3IP1 transgenic plants. The samplings were occurred around noon during the day time. b Starch staining of the control and MdY3IP1 transgenic Arabidopsis plants. c Determination of soluble sugars in the control and MdY3IP1 transgenic Arabidopsis plants. d Chlorophyll content in the leaves of control and MdY3IP1 transgenic Arabidopsis plants. e Maximum quantum yield of PSII (Fv/fm) in the leaves of control and MdY3IP1 transgenic Arabidopsis plants. f Induction and relaxation of NPQ monitored during dark-to-light transition (120 μmol photons m− 2 s− 1). Curves represents an average of six independent measurement. g Immunoblots of PSI core protein subunits including PsaA, PsaD, and PsaF, as well as PSII reaction center protein D1 in the control and MdY3IP1 transgenic Arabidopsis plants. An anti-myc antibody was used to detected protein abundance of MdY3IP1-myc transgenic Arabidopsis. Anti-ACTIN antibody was used as a negative control. Note: In a, c, d, e, data are shown as the mean ± SE, based on more than nine replicates. Statistical significance was determined using Student’s t test. *P < 0.01; **P < 0.001

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