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Fig. 1 | BMC Plant Biology

Fig. 1

From: Ectopic expression of the apple nucleus-encoded thylakoid protein MdY3IP1 triggers early-flowering and enhanced salt-tolerance in Arabidopsis thaliana

Fig. 1

Early flowering phenotype in the MdY3IP1-expressing transgenic Arabidopsis plants. a Subcellular localization of MdY3IP1. pCaMV35S::MdY3IP1-GFP was transiently expressed in protoplasts of apple leaves. pCaMV35S::GFP was used as a negative control. Bars = 100 μm. b Flowering phenotype of the MdY3IP1 transgenic Arabidopsis. 4-week-old plants grown in soil under long days (LDs) were photographed. Note: Arabidopsis plants transformed with an empty vector serve as the control. c Expression of MdY3IP1 in the control and MdY3IP1 transgenic plants by qPCR assay. Note: Arabidopsis plants transformed with an empty vector serve as the control. d, e Determination of leaf number (d) and of days to bolting (e). Approximate 20 plants grown under LDs were counted and averaged in each assay. Note: In d, e, data are shown as the mean ± SE, based on more than nine replicates. Statistical significance was determined using Student’s t test. *P < 0.01; **P < 0.001

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