Analysis of the up-steam regulators of JrVHAG1. a, yeast one-hybrid assay analyses of the upstream regulators of JrVHAG1. p53His2 + pGADT7-p53, positive control, pGADT7-Rec2 vector that encodes murine p53 fused with GAL4 AD; p53His2 + pGADT7-JrMYB2, negative control, pHis2 reportor vector that contains the cis-acting DNA consensus sequence recognized by p53. The transformants spotted on SD/-His/−Leu/−Trp plates with 0 mM 3-AT were used as positive controls for transformant growth. Positive transformants were further confirmed by spotting serial dilutions (1/1, 1/10, 1/100, 1/1000, 1/10000) onto SD/-His/−Leu/−Trp plates with 50 mM 3-AT. b, transient co-transformation of the reportor and effectors into tobacco seedlings, the GUS activities was determined. P, positive control; N, negative control, MYB, M, S, M1, M2 were consistent with a. * means significant differences (p < 0.05) between the negative control and others. c, the expression of JrMYB2 under ABA, CdCl2, ABA+CdCl2 treatments. Every experiment was repeated three times. All data are displayed as the mean ± S.D. of three independent experiments.