Fig. 6
From: Calcium-dependent protein kinases in cotton: insights into early plant responses to salt stress
Expression profile of GhCPK genes in response to salt stress. a The cluster analysis was developed using the K-means method on the expression profiles for a total of 98 CPK genes in G. hirsutum. b qPCR analyses of cluster 4 CPK genes expression following NaCl treatment. Cotton seedlings were irrigated with water containing 0Â mM, 200Â mM or 400Â mM NaCl, respectively. Cotton leaves were collected at 0, 1, 3, 6, and 12Â h after the treatment. The GhUB7 (ubiquitin 7, Accession: DQ116441) gene was used as an internal control. The error bars indicate the standard deviation estimated from the four replicates