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Fig. 2 | BMC Plant Biology

Fig. 2

From: Functional characterization of chitin-binding lectin from Solanum integrifolium containing anti-fungal and insecticidal activities

Fig. 2

Determination of lectin purified from eggplant. a Results of SDS-PAGE analysis of crude extracts and eluted proteins with chitin bead purification. b The reaction mixtures with 2-ME (0, 3, 10, and 30%) were analyzed by non-reducing SDS-PAGE and stained with Coomassie Brilliant Blue. c Deglycosylation of purified lectin. Purified CBL was treated with or without trifluoromethanesulfonic acid (TFMS) and peptide-N-glycosidase F (PNGase F) and then analyzed by SDS-PAGE. The arrow indicates deglycosylation form. d Amino acid sequence obtained from matrix-assisted laser desorption ionization (MALDI) mass spectrometry. Comparison nucleotide sequence with deduced amino acid sequence by EMBOSS prettyseq (http://www.bioinformatics.nl/cgi-bin/emboss/prettyseq)

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BMC Plant Biology

ISSN: 1471-2229

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