Fig. 2

Lignification of the hemp hypocotyl between 6 and 20 days. a to d, fixed cross sections of H6 (a), H9 (b), H15 (c) and H20 (d) stained with FASGA. Lignification of the bast fibres is illustrated in the insets of c and d. e to h, fresh cross-sections of H15 (e and g) and H20 (f and h) stained with Mäule reagent. Higher magnifications of the bast fibres are shown in g and h. The primary xylem cells and tracheary elements of the secondary xylem are indicated with arrow heads, while fibres of the secondary xylem are indicated with an arrow in e and f. Scale bar = 100 μm in the main pictures and 25 μm in the insets