Fig. 1

Overexpression phenotypes of the SAUR8, SAUR10 and SAUR16 overexpression lines. a-b Whole-plant phenotypes of representative Col-0 and 35S:SAUR8/10/16 overexpression lines. Because the phenotypes from the three different constructs were comparable (there is more variation between lines from the same construct than between constructs), only two representative plants are displayed here. The overexpression lines display wavy main stems and side branches, show reduced fertility due to the long pistil size, and have an irregular phyllotaxy. a Col-0 and 35S:SAUR10#4 at 10 days after bolting (DAB). b Col-0 and 35S:SAUR8#2 at 16 days after bolting (DAB). c The siliques of the different overexpression lines are significantly longer than wild-type siliques and have ‘bumpy shoulders’. O16^DST = 35S:SAUR16 including the DST region; OE8/10/16 = 35S:SAUR8/10/16 all OE8/10/16 lines showed a similar silique phenotype, a few representative siliques are shown. d Quantification of silique length in the different overexpression lines. From each construct, The lines with the longest siliques were selected for quantification. O16^DST#6 = 35S:SAUR16DST line 6; OE8#2 = 35S:SAUR8 line 2; etc. e-i Various organs are longer in the SAUR overexpression lines. The phenotypes from the different constructs were very similar, with more variation between the lines of the same construct than between different constructs, and are therefore noted as OE8/10/16 (35S:SAUR8, 35S:SAUR10 or 35S:SAUR16). The phenotypes from the strongest lines are shown. e Length of the sepals of stage 13 flowers. f Length of etiolated hypocotyls 7 days after stratification. g Length of the pistils of stage 13 flowers. h Length of the filaments of stage 13 flowers. i Cauline leaf length and shape. j Expression of the SAUR10-clade genes in the stem of 35S:SAUR10 lines, relative to the expression in wild-type stems. Significant differences (T-test, p < 0.05) are indicated with an asterisk