Table 1 The expression patterns of selected genes in plants under non-filtered air (NF) or elevated O3 (E-O3) using real-time quantitative RT-PCR and RNA-Seq
Gene | Relative expression of the target gene by qRT-PCR | FPKM value from RNA-Seq | Gene annotation | ||
|---|---|---|---|---|---|
| Â | NF | E-O3 | NF | E-O3 | Â |
Bra003517 | 1.07 ± 0.08 b | 2.20 ± 0.26 a | 0 | 7.74 | glutaredoxin family protein |
Bra013923 | 1.12 ± 0.32 b | 85.57 ± 9.21 a | 0 | 5.05 | XTR9 (XYLOGLUCAN ENDOTRANSGLYCOSYLASE 9); hydrolase, acting on glycosyl bonds/ hydrolase, hydrolyzing O-glycosyl compounds/ xyloglucan:xyloglucosyl transferase |
Bra020878 | 1.54 ± 0.58 b | 93.07 ± 10.02 a | 0 | 5.90 | basic helix-loop-helix (bHLH) family protein |
Bra028899 | 1.08 ± 0.07 b | 16.48 ± 4.00 a | 0 | 12.79 | transcription factor |
Bra031485 | 0.98 ± 0.12b | 26.54 ± 2.01 a | 20.40 | 1091.02 | oxidoreductase |
Bra038089 | 0.70 ± 0.26 b | 743.15 ± 58.96 a | 3.07 | 329.91 | lipid binding/ structural constituent of cell wall |
Bra034061 | 0.94 ± 0.13 b | 32.32 ± 5.79 a | 58.71 | 1332.50 | ATGSTU8 (GLUTATHIONE S-TRANSFERASE TAU 8); glutathione transferase |
Bra035732 | 2.23 ± 1.16 b | 9.17 ± 2.00 a | 5.95 | 85.43 | AP2 domain-containing transcription factor, putative |
Bra010802 | 1.16 ± 0.15 b | 4.74 ± 0.94 a | 2.48 | 26.20 | ATGA2OX2 (GIBBERELLIN 2-OXIDASE); gibberellin 2-beta-dioxygenase |
Bra012938 | 1.47 ± 0.58 b | 6.45 ± 0.66 a | 30.81 | 318.51 | ERF104; ethylene-responsive element-binding family protein |
Bra009445 | 0.78 ± 0.33 b | 100.98 ± 38.70a | 0 | 69.96 | KCS19 (3-KETOACYL-COA SYNTHASE 19); acyltransferase/ catalytic/ transferase, transferring acyl groups other than amino-acyl groups |
Bra025833 | 0.95 ± 0.07 a | 0.30 ± 0.03 b | 5.92 | 0 | CAT1 (CATALASE 1); catalase |