Table 1 Sequence of tails and Universal probes (UPs) and an example of Gene specific primers (GSPs), used in Amplifluor-like SNP markers
From: Advantages of Amplifluor-like SNP markers over KASP in plant genotyping
Name | Sequence (5′-3′) |
|---|---|
A. Tails | |
Tail 1-(FAM) | GAAGGTGACCAAGTTCATGCT |
Tail 2-(HEX/VIC) | GAAGGTCGGAGTCAACGGATT |
Tail 3-(FAM) | GTTCATGCT |
Tail 4-(HEX/VIC) | GAACGGATT |
B. Universal probes (UPs) | |
UP-1 (FAM) | FAM-AGCGATGCGTTCGAGCATCGC(T*) GAAGGTGACCAAGTTCATGCT |
UP-2 (HEX/VIC) | HEX/VIC-AGGACGCTGAGATGCGTCC(T*) GAAGGTCGGAGTCAACGGATT |
UP-3 (FAM) | FAM-AGCGATGCGTTCGAGCATCGC(T*) GTTCATGCT |
UP-4 (HEX/VIC) | HEX/VIC-AGGACGCTGAGATGCGTCC(T*) GAACGGATT |
C. Gene-specific primers (Example of barley KATU37, Contig ABC08184) | |
KATU37-F1 | GAAGGTGACCAAGTTCATGCTATTGAGCGATTACGACGAGC |
KATU37-F2 | GAAGGTCGGAGTCAACGGATTATTGAGCGATTACGACGAGA |
KATU37-R | GCAAGGACAAGACAGGCAG |
KATU37-F3 | GTTCATGCTATTGAGCGATTACGACGAGC |
KATU37-F4 | GAACGGATTATTGAGCGATTACGACGAGA |
KATU37-R | GCAAGGACAAGACAGGCAG |