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Fig. 4 | BMC Plant Biology

Fig. 4

From: Advantages of Amplifluor-like SNP markers over KASP in plant genotyping

Fig. 4

Comparison of different concentrations of Taq-polymerase in PCR with Amplifluor-like SNP markers for ‘trouble-shooting’ of allele discrimination. The identicial PCR protocol was applied with the same DNA samples from a wheat collection from Kazakhstan and the same marker W58, using 0.5 units (a) or 0.1 units (b) of Maxima Hot-start Taq-polymerase (ThermoFisher, USA). X- and Y-axes show Relative amplification units, ΔRn, for FAM and VIC fluorescence signals, respectively, as determined by the qPCR instrument

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