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Fig. 3 | BMC Plant Biology

Fig. 3

From: Advantages of Amplifluor-like SNP markers over KASP in plant genotyping

Fig. 3

Comparison of Amplifluor-like SNP marker application with the same UPs labelled with FAM and VIC fluorophores. Quant Studio-7 Real-Time PCR instrument (ThermoFisher Scientific, Paisley, UK), designed for FAM and VIC scoring (a) and Real-Time qPCR system, Model CFX96 (BioRad, Gladesville, NSW, Australia), designed for FAM and HEX discrimination (b), were used. Automatic calls for alleles are shown in different colours, using supplementary software for each instrument separately. X- and Y-axes show Relative amplification units, ΔRn, for FAM and VIC fluorescence signals (a); and Relative fluorescence units, RFU, for FAM and HEX (b); as determined by the qPCR ThermoFisher and BioRad instruments, respectively

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