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Table 2 Primers for ITS1–5.8S–ITS2 region and matK gene

From: Taxonomic assessment of Allium species from Kazakhstan based on ITS and matK markers

Primers Nucleotide sequence Annealing temperature Amplicon sizes
ITS1nF
ITS4nR
5’-AGAAGTCGTAACAAGGTTTCCGTAGG- 3′
5’-TCCTCCGCTTATTGATATGC- 3’
58 °C 638 bp
matK-F
matK-R
5’-CCTATCCATCTGGAAATCTTAG- 3′
5’-GTTCTAGCACAAGAAAGTCG- 3’
50 °C 788 bp