Table 2 Primers for ITS1–5.8S–ITS2 region and matK gene
From: Taxonomic assessment of Allium species from Kazakhstan based on ITS and matK markers
Primers | Nucleotide sequence | Annealing temperature | Amplicon sizes |
|---|---|---|---|
ITS1nF ITS4nR | 5’-AGAAGTCGTAACAAGGTTTCCGTAGG- 3′ 5’-TCCTCCGCTTATTGATATGC- 3’ | 58 °C | 638 bp |
matK-F matK-R | 5’-CCTATCCATCTGGAAATCTTAG- 3′ 5’-GTTCTAGCACAAGAAAGTCG- 3’ | 50 °C | 788 bp |