Fig. 7

Up-regulated genes in a 5mARF17/WT plant. a Panther Go-Slim protein classes of up-regulated genes in 5mARF17/WT plants compared with those in WT. The total hits included 316 genes. The percentage of each term is the clustered gene number of the total protein class hits: calcium binding (5 genes), cell adhesion molecule (3 genes), chaperone (13 genes), cytoskeletal protein (22 genes), defense immunity protein (1 gene), enzyme modulator (12 genes), hydrolase (30 genes), isomerase (5 genes), ligase (15 genes), lyase (6 genes), membrane traffic protein (6 genes), nucleic acid binding (77 genes), oxidoreductase (20 genes), receptor (7 genes), signaling molecule (8 genes), storage protein (3 genes), structural protein (1 gene), transcription factor (18 genes), transfer/carrier protein (11 genes), transferase (26 genes), transmembrane receptor regulatory/adaptor protein (1 gene), and transporter (26 genes). b-l The phenotypes of promoterA9::AT1G48640 and CAMV35s::ZF1 plants. b Quantitative RT-PCR analysis of AT1G48640 expression in WT, 5mARF17/WT and promoterA9::AT1G48640/WT buds. c A 35-day-old promoterA9::AT1G48640/WT plant. Bar = 2 cm. d and e Alexander’s staining of the anthers and pollen grains of a promoterA9::AT1G48640/WT plant. Bars = 20 μm. f Quantitative RT-PCR analysis of ZF1 expression in WT, 5mARF17/WT and CAMV35s::ZF1/WT buds. g A 35-day-old CAMV35s::ZF1/WT plant. Bar = 2 cm. h and i Alexander’s staining of the anthers and pollen grains of a CAMV35s::ZF1/WT plant. Bars = 20 μm. j-l Scanning electron microscopy (SEM) showing the pollen wall structures of a WT plant (j), a promoterA9::AT1G48640/WT plant (k) and a CAMV35s::ZF1/WT plant (l). Bars = 5 μm. The levels of AT1G48640 and ZF1 were normalized to those of tubulin and compared with those of WT. Error bars indicate SD and were calculated from three biological replicates