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Fig. 8 | BMC Plant Biology

Fig. 8

From: Functional analysis of the GRMZM2G174449 promoter to identify Rhizoctonia solani-inducible cis-elements in maize

Fig. 8

Mutation analysis of the GCTGA and TATAT elements in NB leaves. a GFP fluorescence assay of NB leaves expressing different GCTGA mutations after treatment with R. solani strain YWK196 for 24 h. Bars = 5 mm. b Quantitative fluorometric assay of NB leaves. The significant differences were compared with GCTGA. Asterisks indicate statistically significant differences, as determined by Student’s t-tests (*P < 0.05, **P < 0.01). c Conserved motif according to the GCTGA mutation analysis. N = A/T/C/G, H = A/T/C, W = A/T. d GFP fluorescence assay of NB leaves expressing different TATAT mutations after treatment with R. solani strain YWK196 for 24 h. Bars = 5 mm. e Quantitative fluorometric assay of NB leaves. The significant differences were compared with TATAT. Asterisks indicate statistically significant differences, as determined by Student’s t-tests (*P < 0.05, **P < 0.01). f Conserved motif according to the TATAT mutation analysis. D = A/T/G, W = A/T, Y = T/C

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