Fig. 5

The GCTGA and TATAT are two R. solani-inducible cis-elements. a Schematic diagram of the −574 to −550 (I), −490 to −478 (IV) and corresponding deleted derivatives (II, III, V, VI, VII) used to express GFP in NB leaves. b GFP fluorescence assay of young and expanded symmetrical NB leaves infiltrated with the six constructs after R. solani strain YWK196 infection for 24 h. Bars = 5 mm. c Quantitative fluorometric assay of NB leaves. Fluorescence value was calculated relative to CK of I. The 35S minimum promoter was used as the negative control. Asterisks indicate statistically significant differences, as determined by Student’s t-tests (*P < 0.05)