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Table 2 Analysis of proteins co-immunoprecipitated with PETD-cytochrome b 6 complexes after insertion into thylakoid membranes

From: Chloroplast PetD protein: evidence for SRP/Alb3-dependent insertion into the thylakoid membrane

No. Proteina Protein Scoreb Peptide identified by MSc Annotation in database
1 PetB 480 LEIQAIADDITSK
VYLTGGFK
IVTGVPDAIPVIGSSVVELLR
AIK21467
2 PetD 628 KPDLTDPVLRAK
LLGVLLMVSVPAGLLTVPFLENVNK
FQNPFR
RPVATTVFLIGTVVALWLGIGATLPIEK
AAD41889
3 CCB1 225 IVNKTFVK
LLDEVGNKAPNQVAGEVLSFFTR
EDGTLSEIVVQGDDQQVEQMRK
Pisum_sativum_v1_Contig2650 [64]
4 CCB3 99 LMILADLDPATAK
FPYVIAYAPTEPLLVPTRK
Pisum sativum_csfl_reftransV1_0082495 [64]
5 GUFP 43 VIASEALSAIR Q9FNM5
6 GAPDH 117 TFAEEVNEAFR
ELGIDLVIEGTGVFVDR
CAA33264
  1. For the immunoprecipitation of complexes, antibody against cytochrome b 6 was used. Bound proteins were directly analyzed by MS with PMF (data shown in Additional file 6: Table S2
  2. aChloroplast proteins which produced the highest scores are shown
  3. bOthers proteins with higher scores than 40 were not observed after cross-linking
  4. cPeptide identifications were accepted if they could be established at greater than 80.0% probability. Protein shown are from the double expression experiments. Analyses were repeated at least twice. MASCOT search and protein identification criteria were previously published in [11, 28, 36]