Chloroplast PetD protein: evidence for SRP/Alb3-dependent insertion into the thylakoid membrane

BMC Plant Biology

Table 2 Analysis of proteins co-immunoprecipitated with PETD-cytochrome b ₆ complexes after insertion into thylakoid membranes

No.	Protein^a	Protein Score^b	Peptide identified by MS^c	Annotation in database
1	PetB	480	LEIQAIADDITSK VYLTGGFK IVTGVPDAIPVIGSSVVELLR	AIK21467
2	PetD	628	KPDLTDPVLRAK LLGVLLMVSVPAGLLTVPFLENVNK FQNPFR RPVATTVFLIGTVVALWLGIGATLPIEK	AAD41889
3	CCB1	225	IVNKTFVK LLDEVGNKAPNQVAGEVLSFFTR EDGTLSEIVVQGDDQQVEQMRK	Pisum_sativum_v1_Contig2650 [64]
4	CCB3	99	LMILADLDPATAK FPYVIAYAPTEPLLVPTRK	Pisum sativum_csfl_reftransV1_0082495 [64]
5	GUFP	43	VIASEALSAIR	Q9FNM5
6	GAPDH	117	TFAEEVNEAFR ELGIDLVIEGTGVFVDR	CAA33264

For the immunoprecipitation of complexes, antibody against cytochrome b ₆ was used. Bound proteins were directly analyzed by MS with PMF (data shown in Additional file 6: Table S2
^aChloroplast proteins which produced the highest scores are shown
^bOthers proteins with higher scores than 40 were not observed after cross-linking
^cPeptide identifications were accepted if they could be established at greater than 80.0% probability. Protein shown are from the double expression experiments. Analyses were repeated at least twice. MASCOT search and protein identification criteria were previously published in [11, 28, 36]

ISSN: 1471-2229