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Fig. 7 | BMC Plant Biology

Fig. 7

From: Mitochondrial pyruvate carrier 1 mediates abscisic acid-regulated stomatal closure and the drought response by affecting cellular pyruvate content in Arabidopsis thaliana

Fig. 7

Pyruvate enhanced ROS production. a Exogenous ABA alone or pretreatment with ABA and exogenous pyruvate induced ROS production (labeled with CM-H2DCFDA) in guard cells. Bar: 10 μm. b The intensity of the fluorescence signal was measured using ImageJ. Values are means ± SE (n = 3). At least 30 guard cells were measured within each replicate. Statistically significant (P < 0.05) differences are indicated by different letters. c Pyruvate promoted stomatal closure in Col-0, but not the rbohD/F mutant. Values are means ± SE (n = 3). Before width/length ratio analysis, at least 30 stomata were measured. d Patch clamp whole-cell recordings of the anion currents in Col-0, rbohD/F protoplast guard cells in the presence/absence of 100 μM pyruvate. e Current/voltage relationships of whole-cell slow-type anion currents, as illustrated in (d). The numbers of guard cells measured were as follows: Col-0, n = 8; Col-0+Pyruvate, n = 10; rbohD/F, n = 7; rbohD/F+Pyruvate, n = 7. Values are means ± SE

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