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Fig. 4 | BMC Plant Biology

Fig. 4

From: Mitochondrial pyruvate carrier 1 mediates abscisic acid-regulated stomatal closure and the drought response by affecting cellular pyruvate content in Arabidopsis thaliana

Fig. 4

AtMPC1 and NRGA1 formed a heterocomplex. a In vivo co-immunoprecipitation assay revealed an interaction between AtMPC1 and NRGA1 in N. benthamiana leaves. IP: Immunoprecipitation; IB: Immunoblotting. b Identification of mpc1/nrga1 based on qPCR. c The rate of water loss from detached leaves of the double mutant. d Stomatal movement in the double mutant in response to treatment with 50 μM ABA and images of the rosettes of Col-0, mpc1, nrga1, and mpc1/nrga1 at the age used for the stomatal aperture experiments. Values are means ± SE (n = 3). Before width/length ratio analysis, at least 30 stomata were measured

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