Fig. 3

Characterization of EgMYB4. a The conserved amino acids, tryptophan (W) and phenylalanine (F) are indicated by arrows. The R2 and R3 domain is indicated by colored lines on top. The C1 (llsrGIDPxTHR) and C2 (pdLNL[D/E]L) motifs are highlighted with red boxes. b Phylogenetic relationships between EgMYB4 and other characterized R2R3-MYBs of subgroup 4. The tree was constructed using MEGA software. Amino acid sequences were used and bootstrap value was set to 1000 replicates. Selected protein accession numbers are listed in Additional file 5. c Transcript levels (± SE, n = 4) of EgMYB4 in different tissues of oil palm. Samples from different floral stages were collected based on the description of Fig. 2b. Transcript levels were analyzed by qRT-PCR. UBQ: Ubiquitin gene. d Subcellular localization of EgMYB4. N. benthamiana leaves were transformed with Agrobacterium carrying either YFP or EgMYB4-YFP. After 48 h of incubation, the transformed cells were observed under a confocal microscope. Nuclei in leaf epidermal cells were stained with DAPI. Scale bar, 10 μM