Fig. 2

SA accumulation and PR gene expression in the AtELP3 and AtELP4 transgenic F. vesca plants. Free SA levels (top) as well as FvPR1 (middle) and FvPR2 (bottom) expression levels in three AtELP3 (E3) and two AtELP4 (E4) transgenic lines and the non-transformed control (Cont.). SA levels were measured by HPLC and PR gene expression was analyzed by RT-qPCR. Data represent the average of three biological replicates with SD. Expression of PR genes was normalized against the constitutively expressed EF1α gene. An asterisk indicates significant difference between the transgenic line and the non-transformed control (Student’s t-test, p < 0.05). The experiments were repeated three times with similar trends