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Fig. 2 | BMC Plant Biology

Fig. 2

From: Comprehensive transcriptome analysis reveals distinct regulatory programs during vernalization and floral bud development of orchardgrass (Dactylis glomerata L.)

Fig. 2

Transcriptional relationship between samples. a Number of up- and down-regulated genes in five pairwise sampling stages, including stage V_DON vs stage BV_DON (V vs BV), stage AV_DON vs stage V_DON (AV vs V), stage VG_DON vs stage AV_DON (VG vs AV), stage BH_DON vs stage VG_DON (BH vs VG), and stage H_DON vs stage BH_DON (H vs BH). b Principal component analysis based on all expressed genes, showing six distinct groups of samples: BV_DON_rep1, BV_DON_rep2, BV_DON_rep3 (light green circle); V_DON_rep1, V_DON_rep2, V_DON_rep3 (blue regular triangle); AV_DON_rep1, AV_DON_rep2, AV_DON_rep3 (brown cross); VG_DON_rep1, VG_DON_rep2, VG_DON_rep3 (purple cross); BH_DON_rep1, BH_DON_rep2, BH_DON_rep3 (khaki rhombus), H_DON_rep1, H_DON_rep2, H_DON_rep3 (green inverted triangle). c Cluster dendrogram, showing the global relationship between biological replicates. The y-axis shows the degree of variance. d Number of expressed unigenes and respective expression levels in each sample type, based on the FPKM of biological replicates. Sample labels are as follows: BV, before vernalization; V, vernalization; AV, after vernalization; VG, vegetative growth; BH, before heading; H, heading. DON refers to the orchardgrass cultivated varity DONATA (Registered No.398)

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