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Table 3 Thermocycling conditions for amplification of genes using the PCR

From: Phylogeny and maternal donors of Elytrigia Desv. sensu lato (Triticeae; Poaceae) inferred from nuclear internal-transcribed spacer and trnL-F sequences

Gene Protocol
nrITS 1 cycle: 3 min 94 °C; 35 cycles: 1 min 94 °C, 1 min 52 °C, 1 min 72 °C; 1 cycle: 8 min 72 °C
trnL-F 1 cycle: 4 min 94 °C; 25 cycles: 40 s 94 °C, 50 s 60 °C, 2 min 72 °C; 1 cycle: 8 min 72 °C