An optimized protocol for the preparation of oxygen-evolving thylakoid membranes from Cyclotella meneghiniana provides a tool for the investigation of diatom plastidic electron transport

Table 1 Oxygen evolution of intact cells and isolated thylakoid membranes of C. meneghiniana. FeCN (2 mM) or DCBQ (0.5 mM) were used as electron acceptors, NH₄Cl (10 mM) as uncoupler of the light-driven proton gradient. The Chl concentration of the cells or thylakoid membranes for the oxygen evolution measurements was 10 μg mL⁻¹

Sample	Electron acceptor	Oxygen evolution [μmol O₂ mg Chl⁻¹ h⁻¹]
Untreated thylakoids	DCBQ	117.44 ± 19.8
Untreated thylakoids	FeCN; FeCN, −NH₄Cl	29.48 ± 4.31; 23.43 ± 4.47
Shocked thylakoids	DCBQ	101.12 ± 9.84
Shocked thylakoids	FeCN; FeCN, −NH₄Cl	29.75 ± 6.7; 21.1 ± 1.02
Cells	No supplement	162.67 ± 28.99

For further information about the measurement conditions see the Methods section. Table 1 depicts the mean values of three independent thylakoid isolations with the respective standard deviations

ISSN: 1471-2229