Fig. 7From: An optimized protocol for the preparation of oxygen-evolving thylakoid membranes from Cyclotella meneghiniana provides a tool for the investigation of diatom plastidic electron transportTime-course of NPQ of isolated thylakoids during illumination with 1.000 μmol m−2 s−1 for 25 min in the presence of 30 mM ascorbate and 2 mM FeCN (HL). 3 mM DTT was used to inhibit DD de-epoxidation (HL + DTT). The illumination was performed at room temperature with a Chl concentration of 10 μg mL−1. NPQ is calculated as (Fm/Fm ′-1). The measurements were done with three independent thylakoid preparations, Fig. 7 shows a representative measurementBack to article page