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Fig. 7 | BMC Plant Biology

Fig. 7

From: An optimized protocol for the preparation of oxygen-evolving thylakoid membranes from Cyclotella meneghiniana provides a tool for the investigation of diatom plastidic electron transport

Fig. 7

Time-course of NPQ of isolated thylakoids during illumination with 1.000 μmol m−2 s−1 for 25 min in the presence of 30 mM ascorbate and 2 mM FeCN (HL). 3 mM DTT was used to inhibit DD de-epoxidation (HL + DTT). The illumination was performed at room temperature with a Chl concentration of 10 μg mL−1. NPQ is calculated as (Fm/Fm -1). The measurements were done with three independent thylakoid preparations, Fig. 7 shows a representative measurement

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