Profiling membrane glycerolipids during γ-ray-induced membrane injury

Zheng, Guowei; Li, Weiqi

doi:10.1186/s12870-017-1153-9

BMC Plant Biology

Table 1 Total content of each lipid class after γ-ray treatment at different doses in WS, PLDδ-deficient (def), Col and PLDα1-def plants

From: Profiling membrane glycerolipids during γ-ray-induced membrane injury

Lipid species	Genotype	Dose of gamma irradiation (Gy)
		0	46	210	1100	RC (%)
		Lipid content (nmol per mg dry weight)
DGDG	WS	29.90 ± 1.11^aA	31.67 ± 3.23^aA	18.08 ± 1.20^bA	10.05 ± 1.52^cA	−66.39
	PLDδ-def	28.61 ± 1.90^aA	31.05 ± 4.47^aAB	14.27 ± 2.94^bB	11.18 ± 2.25^bA	−60.92
	Col	22.87 ± 2.55^aB	25.70 ± 2.53^aB	13.86 ± 0.91^bB	9.60 ± 1.65^cA	−58.02
	PLDα1-def	24.20 ± 5.25^aB	26.71 ± 1.60^aB	13.71 ± 1.10^bB	8.40 ± 1.94^cA	−65.29
MGDG	WS	110.89 ± 7.72^aA	109.24 ± 21.60^aB	67.94 ± 5.03^bA	27.38 ± 3.84^cA	−75.31
	PLDδ-def	131.39 ± 8.28^aA	127.81 ± 9.83^aAB	57.18 ± 16.82^bAB	30.00 ± 9.49^cA	−77.17
	Col	132.29 ± 39.34^aA	135.45 ± 13.70^aAB	49.12 ± 14.42^bAB	22.76 ± 2.31^bA	−82.80
	PLDα1-def	131.57 ± 12.34^bA	148.51 ± 14.34^aA	44.73 ± 4.67^bB	6.31 ± 1.54^cB	−95.20
PG	WS	6.47 ± 0.68^aB	6.39 ± 0.89^aC	5.04 ± 0.32^bA	2.18 ± 0.26^cA	−66.31
	PLDδ-def	9.16 ± 1.59^aA	8.48 ± 0.95^aB	4.39 ± 1.55^bAB	2.45 ± 0.66^cA	−73.25
	Col	8.28 ± 2.10^aAB	8.95 ± 0.12^aAB	3.77 ± 0.04^bAB	2.06 ± 0.35^cAB	−75.12
	PLDα1-def	9.88 ± 0.54^aA	9.76 ± 0.29^aA	3.37 ± 0.42^bB	1.48 ± 0.44^cB	−85.02
PA	WS	0.21 ± 0.02^abA	0.28 ± 0.21^aA	0.06 ± 0.03^bcA	0.04 ± 0.03^cA	−80.95
	PLDδ-def	0.16 ± 0.06^aAB	0.20 ± 0.05^aA	0.05 ± 0.01^bAB	0.05 ± 0.07^bA	−68.75
	Col	0.15 ± 0.03^aB	0.13 ± 0.02^aA	0.03 ± 0.02^aAB	0.24 ± 0.34^aA	60.00
	PLDα1-def	0.07 ± 0.04^aC	0.19 ± 0.08^aA	0.03 ± 0.01^aB	1.57 ± 1.31^aA	2142.86
PC	WS	12.02 ± 1.01^aB	12.34 ± 2.50^aB	9.84 ± 2.67^aA	9.85 ± 0.86^aB	−18.05
	PLDδ-def	14.20 ± 0.78^aAB	15.45 ± 0.78^aA	9.36 ± 1.02^bA	10.46 ± 1.13^bB	−26.33
	Col	16.10 ± 4.20^aAB	16.72 ± 0.52^aA	10.12 ± 0.49^bA	13.96 ± 3.02^abA	−13.29
	PLDα1-def	17.84 ± 5.66^aA	16.56 ± 0.59^abA	10.51 ± 0.38^bcA	9.77 ± 3.17^cB	−45.24
PE	WS	2.58 ± 0.32^bB	3.67 ± 1.32^abB	4.69 ± 1.44^aA	4.67 ± 0.40^aA	81.01
	PLDδ-def	3.41 ± 0.97^aB	4.67 ± 1.70^aAB	4.79 ± 0.43^aA	4.41 ± 0.69^aA	29.33
	Col	5.03 ± 0.50^aA	4.57 ± 0.52^aAB	4.35 ± 0.85^aA	4.95 ± 0.66^aA	−1.59
	PLDα1-def	5.61 ± 1.60^aA	5.87 ± 0.71^aA	5.59 ± 1.00^aA	1.83 ± 0.32^bB	−67.38
PI	WS	4.44 ± 0.43^aC	4.54 ± 1.10^aA	3.99 ± 0.27^aAB	4.17 ± 0.81^aB	−6.08
	PLDδ-def	5.01 ± 0.12^bAB	5.51 ± 0.05^aA	3.58 ± 0.41^cAB	3.33 ± 0.27^cBC	−33.53
	Col	4.66 ± 0.63^aBC	4.89 ± 0.20^aA	3.33 ± 0.26^bB	5.21 ± 0.91^aA	11.80
	PLDα1-def	5.26 ± 0.27^aA	5.20 ± 0.44^aA	4.22 ± 0.72^bA	3.09 ± 0.46^cC	−41.25
PS	WS	0.37 ± 0.07^abA	0.45 ± 0.23^aA	0.25 ± 0.12^abA	0.18 ± 0.09^bA	−51.35
	PLDδ-def	0.38 ± 0.14^aA	0.54 ± 0.27^aA	0.16 ± 0.10^bAB	0.16 ± 0.08^bA	−57.89
	Col	0.25 ± 0.11^abA	0.34 ± 0.01^aA	0.11 ± 0.04^cB	0.19 ± 0.03^bcA	−24.00
	PLDα1-def	0.39 ± 0.11^aA	0.36 ± 0.04^aA	0.12 ± 0.02^bB	0.09 ± 0.03^bA	−76.92
Total	WS	167.31 ± 8.67^aA	168.94 ± 28.87^aB	111.77 ± 5.48^bA	56.29 ± 3.57^cA	−66.36
	PLDδ-def	193.47 ± 11.35^aA	192.12 ± 11.80^aAB	94.64 ± 24.19^bAB	62.21 ± 12.26^cA	−67.85
	Col	189.87 ± 47.98^aA	197.15 ± 15.57^aAB	86.00 ± 10.78^bB	59.78 ± 10.33^bA	−68.52
	PLDα1-def	194.49 ± 24.18^aA	214.50 ± 15.16^aA	79.35 ± 3.34^bB	34.55 ± 5.80^cB	−82.24

The RC is the percentage value of the 1100-Gy-treated plants compared with the control (0 Gy). Different lowercase letters marked in the same row represent significantly different among different γ-ray treatments. Different uppercase letters marked in the same column represent significantly different among different genotypes, (p < 0.05). Data are means ± standard deviation (n = 4 or 5)

Back to article page

ISSN: 1471-2229

Contact us

General enquiries: journalsubmissions@springernature.com