Fig. 4
From: Plant-mediated RNAi silences midgut-expressed genes in congeneric lepidopteran insects in nature
Silencing of MqCYP6B46 and MqBG1does not silence the most closely related, likely non-target genes. a Alignment of MqCYP6B46 partial coding sequence with MqCYP6B45 showed 83% sequence similarity and one identical homologous region >21 nt was identified. b Alignment of MqBG1 coding sequence with MqBG2 showed 71% sequence similarity and no identical homologous regions >21 nt were identified. Transcripts (relative to ubiquitin) of (c) MqCYP6B45 and (d) MqBG2 in the midguts of fourth-instar EV-, irCYP6B46 and EV-, irBG1-feeding larvae did not show off-target silencing of CYP6B45 and BG2, respectively, (n = 6) Significant differences between means (± SE) in comparison to EV was determined by one-way ANOVA and Fisher LSD post hoc test