Fig. 8

The mRNA-seq based differentially expressed genes for the Cd-exposed fc1 mutants relative to the Cd-exposed Col-0 wild-type. Two week-old Arabidopsis seedlings were exposed to 0 (−Cd) and 50 μM Cd (+Cd) in the half-strength Hoagland solution. a: GO analysis of specific genes from the 227 up-regulated and 148 down-regulated genes (Figure 8d) in fc1 mutants relative to the wild-type under Cd stress. Genes most related to Cd tolerance are noted with red arrows. b, c: Venn diagrams displaying the genes differentially expressed in Col-0 (+Cd)/Col-0 (−Cd) and fcl (+Cd)/fcl (−Cd) under Cd stress. The presented genes are functionally involved in Cd-induced oxidative stress (b) and Cd transport or detoxification (c). The small ellipses in the big ellipses represent the overlap of the genes between Col-0 and fc1 mutant plants. d, e: Heatmap represents the gene expression levels of individual reactive oxygen species-related genes (d) and heavy metal transport and detoxification genes (e). Some of the genes most related to Cd tolerance were highlighted by red tick off