Fig. 3
From: A cadmium stress-responsive gene AtFC1 confers plant tolerance to cadmium toxicity
Accumulation of O2 −, H2O2 and other oxidants in the wild-type (WT), AtFC1 transgenic lines (35S::FC1) and fc1 mutants of Arabidopsis under Cd stress. Seedlings were grown in the half-strength Hoagland solution for 21 d and transferred to the same culture solution with 50 μM Cd for 1 d. a: Histochemical detection of O2 •− on leaves by NBT staining. b: Histochemical detection of H2O2 on leaves by DAB staining. c, d: H2O2 contents in roots and shoots. e: DCFH-DA fluorescent imaging for endogenous ROS. f: Quantitative analysis of fluorescent density of image of (e). g, h: TBARS contents in roots and shoots. Vertical bars represent standard deviation. Asterisks indicate that the mean values are significantly different between the transgenic plants/mutants and WT (p < 0.05)