RNA-Seq analysis of resistant and susceptible sub-tropical maize lines reveals a role for kauralexins in resistance to grey leaf spot disease, caused by Cercospora zeina

Table 1 Read mapping, alignment and subsequent expression summary for RIL165 and RIL387

Sample^a	Number of quality filtered reads	Number of mapped reads^b	Number of paired alignments	Maximum FPKM^c	Number of expressed genes
RIL165_1	70,769,770	83%	27,884,716	5761	22,558
RIL165_2	62,302,258	75%	21,984,219	6882	22,895
RIL165_3	78,883,890	76%	28,417,469	4868	22,773
RIL387_1	62,399,266	80%	23,331,358	6755	22,633
RIL387_2	63,798,694	78%	23,458,704	8659	22,575
RIL387_3	59,304,854	80%	22,353,073	6349	22,641

^aSamples are numbered per biological replicate
^bSequence reads for each biological replicate were mapped to v2 of the B73 reference genome using TopHat v2.0.9 [103]
^cCufflinks v2.0.2 was used to calculate transcript abundance which is reported as fragments per kilobase pair of exon model per million fragments mapped (FPKM) [33]

ISSN: 1471-2229