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Table 1 Summary of the degree of ultrastructural preservation and resolution of cellular substructures by ChF, PF and HPF

From: Efficient preparation of Arabidopsis pollen tubes for ultrastructural analysis using chemical and cryo-fixation

Cellular Substructure Ultrastructure preservation and resolution
ChF PF HPF
LR white Epon Epon Epon
Cell Wall 2 4 3 5
Plasma membrane 1 3 4 5
Golgi complex 1 3 4 5
Mitochondria 2 4 3 4
Endoplasmic reticulum 3 5 5 5
Ribosomes 4 5 5 5
Vacuole 2 3 5 5
Vacuole integration na na 4 5
Vesicles 1 3 4 5
Lipid droplets na 4 2 2
Starch granules 2 4 4 5
Microtubules na na na 5
Sperm cells 3 4 4 5
Nucleus 3 4 4 5
Callose Plugs 4 5 5 5
Cytoplasmic organization 2 4 4 5
Ice crystals damages na na 3 1
  1. The quality of the ultrastructure is rated on a 5 point bases as follows: 1) ultrastructural features unresolvable, the substructure is barely visible; 2) substructure is visible; 3) substructure and some ultrastructural features distinguishable; 4) efficient preservation and resolution of most details of the substructure; 5) fine preservation and resolution of substructure and clear distinction of ultrastructural details, and assumed to be the closest to the native structure. na = not applicable or substructure is not visible