Table 1 Summary of the degree of ultrastructural preservation and resolution of cellular substructures by ChF, PF and HPF
Cellular Substructure | Ultrastructure preservation and resolution | |||
|---|---|---|---|---|
ChF | PF | HPF | ||
LR white | Epon | Epon | Epon | |
Cell Wall | 2 | 4 | 3 | 5 |
Plasma membrane | 1 | 3 | 4 | 5 |
Golgi complex | 1 | 3 | 4 | 5 |
Mitochondria | 2 | 4 | 3 | 4 |
Endoplasmic reticulum | 3 | 5 | 5 | 5 |
Ribosomes | 4 | 5 | 5 | 5 |
Vacuole | 2 | 3 | 5 | 5 |
Vacuole integration | na | na | 4 | 5 |
Vesicles | 1 | 3 | 4 | 5 |
Lipid droplets | na | 4 | 2 | 2 |
Starch granules | 2 | 4 | 4 | 5 |
Microtubules | na | na | na | 5 |
Sperm cells | 3 | 4 | 4 | 5 |
Nucleus | 3 | 4 | 4 | 5 |
Callose Plugs | 4 | 5 | 5 | 5 |
Cytoplasmic organization | 2 | 4 | 4 | 5 |
Ice crystals damages | na | na | 3 | 1 |