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Fig. 6 | BMC Plant Biology

Fig. 6

From: Transposable elements generate population-specific insertional patterns and allelic variation in genes of wild emmer wheat (Triticum turgidum ssp. dicoccoides)

Fig. 6

Examples of site-specific PCR analyses with TE-flanking primers (a, d – genome-specific primers; b, c – primers complementary to both A and B genomes) in 5 populations of wild emmer wheat, showing polymorphic TE insertions. Full and empty sites are indicated by arrows. M denotes size marker. a) Population-unique insertion of Aison 400 bp upstream to TRIUR3_24471 gene, a 564-bp band indicating that a full site is present in all accessions of Mt. Hermon (bottom), 346 bp band indicating that an empty site is present in all accessions of Tabgha and Mt. Amasa populations (top) and of Amiad and Jaba populations (middle). b) Insertion of Minos in exon 2 of the Traes_7AL_0D3EF0026 gene in some accessions of Tabgha (top right) and Amiad (middle right), where the upper band (537 bp) indicates a full site. The lower band (301 bp) is present in all accessions. c) Insertion of Eos in an exon of the Traes_4BS_AAE1439D4 gene, present in all examined accessions of Mt. Amasa (top right) and Mt. Hermon (middle right) populations and some accessions of Amiad (middle left) and Jaba (bottom), but not in Tabgha (top left). The upper band (500 bp) indicates a full site, the lower band present in all accessions (170 bp) indicates an empty site. d) Insertion of Thalos in intron 1 of the TRIUR3_22200 gene present in all accessions Mt. Amasa (top right), Amiad and Mt. Hermon (middle), and absent from one accession of Tabgha (top left) and some accessions of Jaba (bottom). The upper band indicates a full site (347 bp – intact TE, or 295 bp – truncated TE in 2 accessions of Amiad validated by sequencing). The lower band (185 bp) indicates an empty site

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