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Table 2 Primer sequences for the rDNA ITS region and the chloroplast matK gene

From: Morphological description and DNA barcoding study of sand rice (Agriophyllum squarrosum, Chenopodiaceae) collected in Kazakhstan

Primers Nucleotide sequence Annealing temperature Expected sizesa
ITS1nF
ITS4nR
5′-AGAAGTCGTAACAAGGTTTCCGTAGG- 3′
5′-TCCTCCGCTTATTGATATGC- 3′
58 °C 600-680 bp
matK-F
matK-R
5′-CCTATCCATCTGGAAATCTTAG- 3′
5′-GTTCTAGCACAAGAAAGTCG- 3′
50 °C 750-800 bp
  1. aexpected sizes are approximate lengths of amplicons usually obtained from Caryophyllales according to data in GenBank [34]