Morphological description and DNA barcoding study of sand rice (Agriophyllum squarrosum, Chenopodiaceae) collected in Kazakhstan

Table 2 Primer sequences for the rDNA ITS region and the chloroplast matK gene

Primers	Nucleotide sequence	Annealing temperature	Expected sizes^a
ITS1nF ITS4nR	5′-AGAAGTCGTAACAAGGTTTCCGTAGG- 3′ 5′-TCCTCCGCTTATTGATATGC- 3′	58 °C	600-680 bp
matK-F matK-R	5′-CCTATCCATCTGGAAATCTTAG- 3′ 5′-GTTCTAGCACAAGAAAGTCG- 3′	50 °C	750-800 bp

^aexpected sizes are approximate lengths of amplicons usually obtained from Caryophyllales according to data in GenBank [34]

ISSN: 1471-2229