Fig. 8

Detection of S-sulfhydrated proteins. a Protein extracts from 0.25 g of tomato roots were exogenously treated with H₂O (Con), 2 mM Na₂S (for 1 h; another H₂S donor), and 2 mM DTT (for 30 min; a sulfhydration inhibitor), alone or their combinations (treatment with Na₂S followed by DTT), and subjected to the modified biotin switch method (BSM). Finally, the labeled proteins were detected using protein blot analysis with antibodies against biotin. b Three-day-old tomato seedlings were treated with H₂O (Con), 1 mM NaHS, 200 μΜ hypotaurine (HT), and 2 μΜ _DL-propargylglycine (PAG), alone or their combinations for 4 d. Afterwards, protein extracts from 0.25 g of seedling roots were subjected to the BSM, and the labeled proteins were detected using protein blot analysis with antibodies against biotin. Representative pictures were provided