Skip to main content
Fig. 8 | BMC Plant Biology

Fig. 8

From: Hydrogen peroxide is involved in hydrogen sulfide-induced lateral root formation in tomato seedlings

Fig. 8

Detection of S-sulfhydrated proteins. a Protein extracts from 0.25 g of tomato roots were exogenously treated with H2O (Con), 2 mM Na2S (for 1 h; another H2S donor), and 2 mM DTT (for 30 min; a sulfhydration inhibitor), alone or their combinations (treatment with Na2S followed by DTT), and subjected to the modified biotin switch method (BSM). Finally, the labeled proteins were detected using protein blot analysis with antibodies against biotin. b Three-day-old tomato seedlings were treated with H2O (Con), 1 mM NaHS, 200 μΜ hypotaurine (HT), and 2 μΜ DL-propargylglycine (PAG), alone or their combinations for 4 d. Afterwards, protein extracts from 0.25 g of seedling roots were subjected to the BSM, and the labeled proteins were detected using protein blot analysis with antibodies against biotin. Representative pictures were provided

Back to article page
\