Fig. 4

H₂S-induced lateral root primordial (LRP) formation is sensitive to the removal of H₂O₂. Three-day-old tomato seedlings were treated with H₂O (Con), 1 mM NaHS, 100 μΜ H₂O₂, 500 μΜ N,N′-dimethylthiourea (DMTU), and 0.1 μΜ diphenylene idonium (DPI), alone or their combinations. After various treatments for 3 d, photographs showing the representative morphology of LRP (about 75% of LRP at the shown stages), were taken (a). Bar = 0.25 mm. Meanwhile, the number of emerged LRP was also analyzed (b). Mean and SE values were calculated from at least three independent experiments. Bars with different letters denoted significant differences at P < 0.05 according to Duncan’s multiple range test