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Fig. 2 | BMC Plant Biology

Fig. 2

From: Hydrogen peroxide is involved in hydrogen sulfide-induced lateral root formation in tomato seedlings

Fig. 2

H2S-induced H2O2 accumulation is diminished by the scavenger and synthetic inhibitor of H2O2. Three-day-old tomato seedlings were treated with H2O (Con), 1 mM NaHS, 100 μΜ H2O2, 500 μΜ N,N′-dimethylthiourea (DMTU), and 0.1 μΜ diphenylene idonium (DPI), alone or their combinations for 12 h. Afterwards, corresponding confocal images of DCF-dependent fluorescence in seedling roots were provided to represent endogenous H2O2 contents (a), and the relative fluorescence were presented as values relative to Con (b). Scale bar = 200 μm. Meanwhile, the H2O2 contents were determined by spectrophotography (c). Mean and SE values were calculated from at least three independent experiments. Bars with different letters denoted significant differences at P < 0.05 according to Duncan’s multiple range test

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