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Fig. 1 | BMC Plant Biology

Fig. 1

From: Hydrogen peroxide is involved in hydrogen sulfide-induced lateral root formation in tomato seedlings

Fig. 1

Sodium hydrosulfide (NaHS; the H2S donor) increases H2O2 accumulation and thereafter lateral root (LR) formation. Three-day-old tomato seedlings were treated with H2O (Con), 1 mM NaHS, 200 μΜ hypotaurine (HT), and 2 μΜ DL-propargylglycine (PAG), alone or their combinations. After 12 h, the confocal images of AzMC-dependent and DCF-dependent fluorescence in seedling roots were used to represent endogenous H2S (a) and H2O2 (b) contents. Scale bar = 200 μm. Meanwhile, the relative fluorescence presented as values relative to Con (c, d). Also, the emerged LR density and the number of emerged LRs (>1 mm) per seedling (e) were analyzed with plants 4 d after treatments. Mean and SE values were calculated from at least three independent experiments. Within each set of experiments, bars denoted by the same letter did not differ significantly at P < 0.05 according to Duncan’s multiple range test

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