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Fig. 2 | BMC Plant Biology

Fig. 2

From: Constitutive expression of OsDof4, encoding a C2-C2 zinc finger transcription factor, confesses its distinct flowering effects under long- and short-day photoperiods in rice (Oryza sativa L.)

Fig. 2

Subcellular localization of OsDof4. a Subcellular localization of OsDof4 in rice protoplasts. The vector 35S::OsDof4-GFP or 35S::GFP with the nuclear marker plasmids pSAT6-mCherry-VirD2NLS were co-transformed into and transiently expressed in rice protoplasts. Left to right: GFP fluorescence signal, RFP fluorescence signal, Bright-field signal, and Merged signal. Top panel: localization of OsDof4-GFP fusion proteins. Bottom panel: localization of free GFP proteins. Scale bar = 5um. The excitation wavelengths for GFP and RFP detection were respectively 488 nm and 587 nm. b Microscopic observation of the fresh root tips in 40-day-old transgenic plants. The top panel indicated the images acquired from 35S::GFP plants; The bottom panel indicated the images acquired from 35S::OsDof4-GFP plants. Left: GFP fluorescence signal; Right: Merged signal. Bar = 20um

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BMC Plant Biology

ISSN: 1471-2229

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