Table 1 Kinetics of three terpene synthase enzymes from Melaleuca alternifolia leaves
Enzyme | Substrate/cosubstrate | K m |
|---|---|---|
MaTPS-SaH (sabinene hydrate synthase) | GPP | 11 ± 2.0 μM |
MaTPS-Cin (1,8-cineole synthase) | GPP | 31 ± 6.26 μM |
MaTPS-Tln (terpinolene synthase) | GPP | 21 ± 3.18 μM |
MaTPS-Tln (terpinolene synthase) | Mg2+ | 13 ± 0.14 mM |
MaTPS-Tln (terpinolene synthase) | Mn2+ | 0.15 ± 0.01 mM |